Journal: Neuron

Article Title: CD49f is a novel marker of functional and reactive human iPSC-derived astrocytes

doi: 10.1016/j.neuron.2020.05.014

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse neurons were treated with 70% ACM with 5μM IncuCyte Caspase-3/7 Green Apoptosis Assay Reagent (Sartorius; 4440) and imaged every 6 hours for 60 hours on an Incucyte S3 epifluorescence time lapse microscope (Sartorius).

Techniques: Purification, Produced, Recombinant, Marker, Glutamate Assay, Imaging, Apoptosis Assay, Labeling, RNA Sequencing Assay, Transgenic Assay, Expressing, Software, Luminex, Western Blot

Journal: eLife

Article Title: ASIC1a is required for neuronal activation via low-intensity ultrasound stimulation in mouse brain

doi: 10.7554/eLife.61660

Figure Lengend Snippet:

Article Snippet: For ABC-DAB-Nickel staining, tissue sections were first bleached in 1 x TBS containing 0.03% H 2 O 2 for 30 min, and then blocked in TBST (TBS +0.05% Triton X-100) containing 5% bovine serum albumin (BSA) (Sigma-Aldrich, St. Louis, MO, USA) and 5% normal goat serum (NGS from Jackson ImmunoResearch Laboratories, West Grove, PA, USA) at room temperature for 60 min, and incubated with Rabbit polyclonal Phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) primary antibody [(1:500) #9101, Cell Signaling Technology, Danvers, MA, USA] diluted in blocking solution overnight at 4°C.

Techniques: Transfection, Construct, Plasmid Preparation, Control, Avidin-Biotin Assay, Immunohistochemistry, Live Cell Imaging, Staining, Software, Particle Size Analysis

Journal: The FASEB Journal

Article Title: Progenitor recruitment and adipogenic lipolysis contribute to the anabolic actions of parathyroid hormone on the skeleton

doi: 10.1096/fj.201800948RR

Figure Lengend Snippet: PTH reduces BMAT of CR mice. A) Tibias were sectioned and stained with hematoxylin and eosin to observe BMAT. B–E) Marrow fat proportion (B), adipocyte number (C), and size (D) were assessed by using Bioquant software in a region of interest extending down for ∼1.5 mm. The region of interest was located 50 μm down from the growth plate and 50 μm from the cortices. Marrow fat proportion was calculated as the proportion of marrow area occupied by adipocytes. Adipocyte number (Nb.) was calculated as number of adipocyte per square millimeter of marrow area, and adipocyte size represents the average area occupied by each adipocyte. Osteoblast number (E) was quantified on tetrachrome-stained plastic sections of proximal tibias in a region of interest similar to where the adipocytes were counted. Osteoblast number is presented as number of osteoblast per square millimeter of bone surface (BS). *P < 0.05 (n = 3–10/group; 2-way ANOVA).

Article Snippet: Bioquant software (v.18.2.60; Bioquant Image Analysis, Nashville, TN, USA) was used to examine adipocyte numbers and size, count osteoblast numbers, and assess mineralization surface.

Techniques: Staining, Software